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I-PRF: The Next Generation of Platelet-Rich Fibrin for Tissue Regeneration

Facial aging is a complex interplay of intrinsic and extrinsic factors. Intrinsic aging, driven by genetics, leads to a gradual decline in cellular function and extracellular matrix integrity. Extrinsic factors, such as chronic sun exposure, smoking, pollution, and poor diet, significantly accelerate this process. These elements damage skin cells, degrade collagen and elastin, and contribute to hyperpigmentation and telangiectasia. The visible consequences of facial aging manifest as wrinkles, sagging, loss of volume, and altered skin texture. As patient self-perception and social interactions are heavily influenced by appearance, there's a growing focus on safe and effective facial rejuvenation techniques. In recent years, minimally invasive procedures utilizing regenerative medicine principles have gained significant traction, offering a promising alternative to traditional surgical approaches.

The field of aesthetics has seen a surge in injectable treatments using concentrated platelets from a patient's own blood (autologous) to stimulate healing and improve skin appearance. These platelet concentrates contain growth factors that play a key role in processes like new blood vessel formation, cell movement and growth, and collagen production - all important for younger-looking skin.

Early options like Platelet-Rich Plasma (PRP) and Plasma-Rich Growth Factors (PRGF) required adding external substances during blood collection and processing. This raised concerns about potential interference with wound healing.

Platelet-Rich Fibrin (PRF), a newer approach, emerged to address these limitations. Obtained through a single centrifugation step without any additives, PRF is completely natural. It contains various cell types, a fibrin mesh, and growth factors. Depending on the processing method, PRF can be solid (used in oral and maxillofacial surgery) or injectable (i-PRF).

This blog will review the effectiveness and safety of i-PRF for facial rejuvenation using advanced skin analysis and patient-reported outcomes.


Unlike traditional PRF, i-PRF is achieved through a modified centrifugation process that yields a liquid concentrate. This allows for easier injection into facial tissues, potentially promoting a more even distribution of its regenerative properties. Early research suggests promising benefits for facial aesthetics. Compared to standard PRF, i-PRF may boast a higher concentration of leukocytes and platelets, key players in tissue healing and regeneration. Additionally, the fibrin matrix within i-PRF is believed to trap more growth factors, enabling their sustained release and promoting long-term improvements in facial skin texture, wrinkle reduction, and overall appearance. Further clinical studies are needed to fully evaluate the efficacy and safety of i-PRF in facial rejuvenation, but its potential as a minimally invasive, autologous approach is certainly intriguing.


Injectable Platelet-Rich Fibrin (i-PRF) has emerged as a novel approach in regenerative medicine, particularly for applications in facial rejuvenation. This minimally invasive procedure harnesses the body's own healing potential by utilizing concentrated Platelet-Rich Fibrin (PRF), a bioactive scaffold rich in growth factors and cytokines.

The i-PRF procedure is a relatively simple, single-spin process. Peripheral blood is drawn from the patient and collected in specialized tubes without anticoagulants. This crucial step allows for natural platelet activation and subsequent fibrin clot formation. The collected blood then undergoes low-speed centrifugation, carefully separating the red blood cells from the PRF layer. This upper layer, enriched with platelets, leukocytes, and a fibrin matrix, serves as the therapeutic component.

  • Skin Analysis (Pre-Assessment)

Prior to i-PRF treatment, a thorough patient evaluation is crucial. This should include a detailed medical history to identify any potential contraindications, such as uncontrolled bleeding disorders or active infection. Additionally, a comprehensive skin assessment is recommended to determine suitability and potential areas for treatment. This may involve photography and objective evaluation methods like VISIA® analysis. VISIA® offers valuable insights by measuring specific parameters like wrinkles, pigmentation, and texture. However, a doctor's clinical judgment remains paramount when interpreting these findings and tailoring the i-PRF treatment plan to address the patient's individual concerns and realistic expectations.

  • i-PRF Procedure

Preparation of i-PRF involves a few key steps following aseptic techniques.

Step 1 - Blood Collection:

A small volume of whole blood is drawn from the patient.

Step 2 - Centrifugation:

Centrifugation is performed at a lower speed and for a shorter duration than traditional PRF protocols. This step is crucial for isolating a leukocyte- and platelet-rich liquid fraction, with red blood cells left in the pellet.

Step 3 - Optional Activation Step:

Some protocols may incorporate an activation step after centrifugation, using thrombin or calcium chloride. This step stimulates platelet aggregation and growth factor release.

Step 4 - Formation of i-PRF:

The resulting suspension, rich in leukocytes, platelets, and trapped growth factors within a fibrin matrix, constitutes the injectable platelet-rich fibrin (i-PRF) ready for application.

Following topical anesthesia application, the prepared i-PRF is injected into targeted facial regions using a thin needle. Common injection sites include the malar area for volume enhancement, nasolabial folds for wrinkle reduction, and the upper lip for improved texture. The doctor will determine the optimal injection technique and volume distribution based on the patient's needs. Total treatment time is typically brief, and post-procedure instructions are provided to manage potential side effects like mild swelling, bruising, or temporary discomfort. Patients are also advised on sun protection to optimize treatment results.

Source: Injectable platelet-rich fibrin for facial rejuvenation: A prospective, single-center study (2020)

Mechanism of Action

The mechanism of action of i-PRF is multifaceted. The concentrated platelets within the PRF gradually release a plethora of growth factors, including Platelet-Derived Growth Factor (PDGF), Vascular Endothelial Growth Factor (VEGF), and Transforming Growth Factor-β (TGF-β). These factors orchestrate a cascade of cellular events, promoting tissue regeneration, angiogenesis (new blood vessel formation), and extracellular matrix remodeling. Additionally, the fibrin matrix itself serves as a scaffold, providing structural support for cell migration and facilitating sustained growth factor release.

The minimally invasive nature of i-PRF injections, coupled with its autologous origin, minimizes the risk of immunological reactions and disease transmission. Furthermore, the slow and sustained release of growth factors from the PRF matrix offers a potential advantage over other platelet concentrates, promoting prolonged cellular stimulation and potentially enhancing treatment efficacy.

While the clinical applications of i-PRF are still evolving, its potential for facial rejuvenation appears promising. Studies have shown its effectiveness in improving skin texture, reducing wrinkles, and promoting overall facial volumization. However, further research is necessary to fully elucidate the long-term outcomes and optimal treatment protocols for various clinical settings.

Key Techniques to Achieve Optimal Results

Achieving optimal outcomes with i-PRF hinges on several key techniques.

  1. Meticulous Adherence to Aseptic Protocols Ensure strict adherence to aseptic protocols during i-PRF preparation to minimize contamination risk.

  2. Critical Centrifugation Process Unlike PRP, i-PRF utilizes a low-speed centrifugation protocol to preserve leukocytes and growth factors within the final product. This technique allows for a more even distribution of regenerative cells when injected.

  3. Proper Injection Technique The doctor should possess a thorough understanding of facial anatomy to target specific areas like the malar region for volume enhancement or nasolabial folds for wrinkle reduction.

  4. Thin Needle and Multiple Injection Points Using a thin needle and injecting small aliquots at multiple injection points helps achieve a natural-looking result and minimizes the risk of complications.

  5. Crucial Patient Selection Proper patient selection is crucial. i-PRF is most effective for patients with mild to moderate signs of facial aging and realistic expectations. A pre-treatment consultation allows for a thorough medical history review to identify potential contraindications and ensure patient suitability for the procedure.

In conclusion, injectable Platelet-Rich Fibrin (i-PRF) represents a promising minimally invasive approach for facial rejuvenation. By harnessing the body's natural healing potential through concentrated platelets and growth factors, i-PRF offers potential benefits for improving skin texture, reducing wrinkles, and promoting facial volumization. Early research suggests its safety and efficacy, but further studies are needed to fully optimize treatment protocols and assess long-term outcomes. With careful patient selection, meticulous technique, and adherence to aseptic protocols, i-PRF holds promise as a valuable tool in the field of aesthetic medicine.


Injectable platelet-rich fibrin for facial rejuvenation: A prospective, single-center study (2020)

Platelet-rich Plasma use for facial rejuvenation: a clinical trial and review of current literature (2021)

The use of platelet-rich plasma in aesthetic and regenerative medicine: A comprehensive review (2019)

The potential of topical and injectable growth factors and cytokines for skin rejuvenation (2014)

Advanced PRF and i-PRF: Platelet concentrate or blood concentrate? (2014)


Discover the Science behind PRP (Platelet Rich Plasma) & PRF (Platelet-Rich Fibrin) Therapy in our upcoming Mini Fellowship happening globally:

IFAAS Master Class (Hands-On)

Advanced PRP (Platelet Rich Plasma) & PRF (Platelet-Rich Fibrin) Therapy

for Facial, Neck & Hair Rejuvenation

June 22, 2024 - Sydney, Australia - [Register Now]





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