The Fractionation of Adipose Tissue Procedure to Obtain Stromal Vascular Fractions for Regenerative

Autologous adipose tissue transplantation is clinically used to reduce dermal

scarring and to restore volume loss. The therapeutic benefit on tissue damage

more likely depends on the stromal vascular fraction of adipose tissue than on

the adipocyte fraction. This stromal vascular fraction can be obtained by

dissociation of adipose tissue, either enzymatically or mechanical. Enzymatic

dissociation procedures are time-consuming and expensive. Therefore, we

developed a new inexpensive mechanical dissociation procedure to obtain the

stromal vascular fraction from adipose tissue in a time sparing way, which is

directly available for therapeutic injection. This mechanical dissociation

procedure is denoted as the fractionation of adipose tissue (FAT) procedure. The

FAT procedure was performed in eleven patients.

The composition of the FATstromal vascular fraction was characterized by immunohistochemistry. Adipose derived stromal cells isolated from the FAT-stromal vascular fraction were compared with adipose derived stromal cells isolated from nondissociated adipose tissue (control) for their CD-surface marker expression, differentiation and colony forming unit capacity. Case reports demonstrated the therapeutic effect of the FAT-stromal vascular fraction.

The FAT-stromal vascular fraction is an enrichment of extracellular matrix containing a microvasculature and culturable adipose derived stromal cells. Adipose derived stromal cells isolated from FAT-stromal vascular fraction did not differ from adipose derived stromal cells isolated from the control group in CD-surface marker expression, differentiation and colony forming unit capacity. The FAT procedure is a rapid effective mechanical dissociation procedure to generate FAT-stromal vascular fraction ready for injection with all its therapeutic components of adipose tissue: it contains culturable adipose derived stromal cells embedded in their natural supportive extracellular matrix together with the microvasculature.

Read IFAAS Faculty, Dr Jeroen Steven's latest study on" The Fractionation of Adipose Tissue Procedure to Obtain Stromal Vascular Fractions for Regenerative Purposes" and learn how you can utilise the skill to enhance treatment results for your own patients:

Get the latest know how in Stem Cell Therapy by learning from the Inventor of Organic Surgery Concept Dr Jeroen Stevens on PRP + Stroma (SVF) in a 100% closed system with ACP-syringes by registering IFAAS Hands-On Master Class: Stem Cell , PRP & PRS Therapy for Facial Rejuvenation & Sports Medicine that is coming up in May 9-10, 2017 in Switzerland and Nov 13-14, 2017 in Hong Kong.